Xenbase Image ID: 24311

Fig. 4. VP16-Gsc activates Xwnt8 expression in animal explants. Animal explants were isolated from uninjected (A,B) or VP16-Gsc-injected embryos (C,D), and were cultured in the absence (A,C) or presence (B,D) of Activin protein. The morphology of explants was assessed at the tailbud stage. The expression of Xbra, Xwnt8 and BMP4 in response to Gsc or VP16-Gsc was examined by RT-PCR analysis at the gastrula stage (E). EF1α is a control for RNA recovery and loading. Intact embryos (embryo) served as a positive control and an identical reaction without reverse transcriptase controlled for PCR contamination (embryo-RT). Scale bar: 0.2 mm. Image published in: Yao J and Kessler DS (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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