XB-IMG-24312
Xenbase Image ID: 24312
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Fig. 5. Cell autonomous activation of Xwnt8 by VP16-Gsc. At the 16-cell stage, one dorsal marginal zone blastomere was injected with 750 pg of β-galactosidase (β-gal) mRNA (A) or a combination of β-gal and 500 pg of VP16-Gsc (B). Descendents of the injected cell were identified at the gastrula stage by the presence of β-gal, using the Rose-gal substrate. Activation of Xwnt8 by VP16-Gsc in the dorsal marginal zone and endogenous ventrolateral expression of Xwnt8 was detected by in situ hybridization with BM-purple substrate (A,B). Dorsal views of stage 10.25 embryos with endogenous Xwnt8 expression visible in lateral regions. Insets show higher magnification views of boxed regions. Black arrowheads indicate β-gal-positive nuclei (red) and the white arrowhead indicates cytoplasmic Xwnt8 in situ stain (purple). Scale bar: 0.25 mm; 80 μm in the insets. Image published in: Yao J and Kessler DS (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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