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XB-IMG-24327

Xenbase Image ID: 24327

Fig. 3. Expression pattern of neural markers in embryos injected with XMeis3-AM RNA. Two-cell albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 50-100 pg of XMeis3−AM RNA. The red arrow delineates the dorsal midline. In all embryos, XMeis3-AM injection is on the left side. In all cases (except Fig. 3M), embryos are viewed dorsally; embryos are oriented anterior (top) to posterior (bottom). (A) In situ hybridization with otx2. Expression is expanded posteriorly on the XMeis3-AM injected side. The red lines delineate the AP extent of otx2 expression on the uninjected versus injected side. (B) In situ hybridization with otx2 and Krox20. otx2 expression is expanded posteriorly and Krox20 expression (blue arrows) is lost on the XMeis3-AM-injected side. The red lines delineate the AP extent of otx2 expression on the uninjected versus injected side. (C) In situ hybridization with otx2 and En2 (red). Expression of otx2 and En2 (blue arrows) is expanded posteriorly. (D) In situ hybridization with cpl-1 and En2 (red). cpl-1 expression is expanded posteriorly and En2 expression (blue arrow/uninjected side) is lost on the XMeis3-AM-injected side. The red lines delineate the AP extent of cpl-1 expression on the uninjected versus injected side. (E) In situ hybridization with XAG1; expression is expanded posteriorly and laterally on the XMeis3-AM injected side. (F) In situ hybridization with nrp1; expression is unchanged on the XMeis3-AM injected side. (G) In situ hybridization with Krox20 and HoxB9; Krox20 expression (blue arrows/uninjected side) is eliminated on the XMeis3-AM-injected side. HoxB9 expression is unchanged on the XMeis3-AM injected side. (H) In situ hybridization with XE10. XE10 expression is eliminated on the XMeis3-AM-injected side. (I) In situ hybridization with HoxB1. HoxB1 expression is eliminated on the XMeis3-AM injected side. (J) In situ hybridization with HoxB3. HoxB3 expression is eliminated on the XMeis3-AM-injected side. (K) In situ hybridization with En2 (red) and Krox20. En2 expression is pushed posteriorly to the r3/r4 boarder and Krox20 expression is pushed posteriorly to the r5/r7 boarder on the XMeis3-AM-injected side. (L) In situ hybridization with En2 and Krox20. En2 expression is pushed posteriorly to r3 on the XMeis3-AM-injected side. The blue arrow delineates the reduced Krox20 expression (fused stripes) on the injected side. (M) In situ hybridization with En2 (red) and Krox20. Expression of En2 and Krox20 (blue arrows/uninjected side) is eliminated on the XMeis3-AM-injected side. An anterior view of the embryo: dorsal (top) to ventral (bottom). (N) In situ hybridization with n-tubulin. n-tubulin expression is eliminated on the XMeis3-AM-injected side. The trigeminal neuron is marked by blue arrows on both sides.

Image published in: Dibner C et al. (2001)

Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
hoxb3.Shox2.7, hoxb-3X.laevisThroughout NF stage 21hindbrain
neuroectoderm
rhombomere R5
rhombomere R6
en2.Lengrailed homolog 2, En-2, engrailed-2, engrailed 2, eng2, xen2, maben, en2-a, en2-bX.laevisThroughout NF stage 21neuroectoderm
presumptive midbrain-hindbrain boundary
egr2.LXKr20, EGR-2, Krox-20, XKrox-20, krox20X.laevisThroughout NF stage 21cranial neural crest
neuroectoderm
rhombomere R3
rhombomere R5
nullX.laevisThroughout NF stage 21spinal cord
central nervous system
otx2.SXotx2, otx-2, Xotx-2, otxA, otx2-a, otx2-bX.laevisThroughout NF stage 21neuroectoderm
anterior
anterior neural fold
hoxb1.Lhox-2.9, hoxb-1, XHox-b1X.laevisThroughout NF stage 21midbrain
neuroectoderm
rhombomere R4
ctsl.Lmep, catl, cpl-1, cathepsin LX.laevisThroughout NF stage 21anterior neural fold
neuroectoderm
pre-chordal neural plate
ag1.LXAG, xag1, xag-1, ag, Xag2, ag1-a, ag1-bX.laevisThroughout NF stage 21cement gland primordium
epha2.LXE10X.laevisThroughout NF stage 21hindbrain
neuroectoderm
tail region
rhombomere R4
nrp1.LA5 antigen, np-1, neuropilin-1, nrp, npn-1, nrp-1, neuropilin, pNPG152X.laevisThroughout NF stage 21central nervous system
neuroectoderm
tubb2b.Sn-tubulin, ntubulin, Xn-tubulin, N-Tub, neural beta-tubulin, NBT, NSTX.laevisThroughout NF stage 21neuron
trigeminal ganglion
rhombomere R2
meis3.LXMeis3, mrg2, meis3-a, meis3-bX.laevisThroughout NF stage 21hindbrain
spinal cord
anterior

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