Xenbase Image ID: 24330

Fig. 6. XMeis3 antisense morpholino oligonucleotide eliminates hindbrain marker expression. Two-cell albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 6-7.5 ng of the XMeis3 AMO. In situ hybridization was performed in late neurula stage embryos. In all cases, embryos are viewed dorsally; embryos are oriented anterior (top) to posterior (bottom). The red arrow delineates the dorsal midline. Embryos were injected on the right side. (A) In situ hybridization with Krox20 and HoxB9; Krox20 expression is eliminated on the AMO-injected side. HoxB9 expression is unchanged on the AM- injected side. (B) In situ hybridization with XE10; expression is eliminated on the AMO-injected side. (C) In situ hybridization with HoxB3 and HoxB9; HoxB3 expression is eliminated on the AMO-injected side. HoxB9 expression is unchanged on the AMO-injected side. (D) In situ hybridization with XMeis3 and En2 (red); expression of both markers is posteriorized on the AMO-injected side. (E) In situ hybridization with XMeis3; expression is inhibited on the AMO-injected side. The XMeis3 expression in r2 is indicated by arrows on both sides. Image published in: Dibner C et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hoxb3.S hox2.7, hoxb-3, LOC108700804, LOC108702517 X. laevis Throughout NF stage 21 hindbrain neuroectoderm rhombomere R5 rhombomere R6 en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 21 neuroectoderm anterior neural fold egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 21 hindbrain cranial neural crest neuroectoderm rhombomere R3 rhombomere R5 meis3.L meis3-a, meis3-b, mrg2, XMeis3 X. laevis Throughout NF stage 21 hindbrain spinal cord anterior neuroectoderm epha2.L LOC100191024, XE10 X. laevis Throughout NF stage 21 hindbrain neuroectoderm tail region rhombomere R4 Image source: Published Larger Image Printer Friendly View

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