Xenbase Image ID: 2443

Fig. 4. The effect of FrdMO and DprMO on organizer markers. (A) Four-cell stage embryos were injected in the dorsal equatorial region of two blastomeres with morpholinos and mRNAs as indicated in Table 4, were cultured until stage 10.5 and were subjected to whole-mount in situ hybridization with organizer markers, chordin (left panels), Xnr3 (middle panels) and gsc (right panels) as probes. Co-injection of FrdMO and DprMO can reduce chordin and Xnr3 but not gsc, whereas βcatMO strongly inhibited expression of all three genes, implying that Frodo and Dapper function in a gene-specific manner. The reduction of chordin and Xnr3 by co-injection of FrdMO and DprMO was reversed not only by Frodo RNA, but alsoβ -catenin RNA. (B) Four-cell stage embryos were injected in the equatorial region of each blastomere with morpholinos and mRNAs as indicated and were analyzed at stage 10 and 11 by RT-PCR, using primers specific for chordin, cerberus, Xnr3, siamois, gsc and vent1. EF1α primers were used to control loading. Image published in: Hikasa H and Sokol SY (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 10.5 dorsal marginal zone gsc goosecoid, gsc-a, gsc-b, Xgsc Xenopus Throughout NF stage 10.5 dorsal marginal zone nodal3.1 nr3, Xnr-3, Xnr3 Xenopus Throughout NF stage 10.5 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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