XB-IMG-24452
Xenbase Image ID: 24452
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Fig. 1. An analysis of the temporal and spatial expression pattern of derrière transcripts relative to other early markers of mesoderm and endoderm. (A) Analysis of temporal expression patterns by RT-PCR in Xenopus embryos. derrière transcripts are first detected at stage 8.5 at the same time as xnr1 and xnr4 as well as the homeobox gene mix1. Transcription of the mesodermal marker xbra and the dorsal specific marker cerberus are detected soon afterwards. (B-S) Whole-mount in situ hybridization analysis of Xenopus embryos at stages 8.5, 10+ and 11. The upper panels in each row show a representative embryo bisected through the animal-vegetal axis and oriented with the dorsal side on the right. The lower panels show a vegetal view of whole embryos, again oriented with dorsal sides to the right. (B-G) derrière, sox17β and bix4 transcripts are detected in distinct but overlapping domains at stage 8.5. Localized expression of xnr2, xbra and gsc is not apparent at this stage. (H-M) All transcripts show strong localized expression by stage 10+. derrière expression mirrors that of xbra, while xnr2 transcripts are restricted to the superficial cells of the marginal zone and greatly enriched on the dorsal side. (N-S) Expression in stage 11 embryos. Again derrière transcripts are detected throughout the region of the embryo expressing xbra. Arrowheads indicate the location of the dorsal blastopore lip in stage 10+ embryos. Image published in: Eimon PM and Harland RM (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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