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Fig. 11. Inhibition of activin-like or FGF signalling does not interfere with activation of derriã¨re but does prevent its maintenance. Embryos were injected at the two-cell stage with 800 pg RNA encoding the truncated activin receptor î XAR (A,D), the truncated FGF receptor XFD (B,E), or the control construct d50 (C,F). RNA encoding î²-galactosidase was co-injected as a cell lineage label in all cases. Embryos were allowed to develop to stage 9.5 (A-C) or 11 (D-F) and expression of derriã¨re was analysed by in situ hybridisation. The initial activation of derriã¨re is not affected by inhibition of activin-like (A) or FGF (B) signalling; note the overlap of î²-galactosidase staining (blue) with the in situ hybridisation reaction product (purple), which at this early stage is largely nuclear. Continued expression of derriã¨re, however, does require activin-like and FGF signalling; note down-regulation of expression in D and E. Vg, vegetal pole. Image published in: White RJ et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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