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Fig. 11. FGF effect on neuralized animal caps. As shown in the scheme, animal caps were dissected from stage 10.5 embryos injected either with chordin (660 pg)+cer-S (2 ng) mRNA (A-O), or with cerberus mRNA (2 ng) (A′-N′), and recombined in pairs either without addition of FGF-soaked beads (A,F,K,A′,E′,I′,M′) or with FGF-soaked beads (B-E,G-J,L-O,B′-D′,F′-H′,J′-L′,N′). After reaching stage 30/31, they were processed by in situ hybridization for the expression of Xnkx2.1 (A-E,A′-D′), eomes (F-J,E′-H′), Xemx1 (K-O,I′-L′) or Xnkx2.4 (M′,N′). Concentrations used for FGF8 were 100 ng/μl (B,G,L,B′,F′,J′), 200 ng/μl (C,H,M,C′,G′,K′,N′) or 400 ng/μl (D′,H′,L′); concentrations used for bFGF were 100 ng/μl (D,I,N) or 200 ng/μl (E′,J′,O′). Image published in: Lupo G et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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