Xenbase Image ID: 24556

Fig. 6. Inhibition of Snail activity blocks the expression of neural crest markers. One blastomere of a 2-cell stage embryo was injected with 700 pg of the different dominant negative constructs, treated with dexamethasone at stage 12.5, fixed at stage 19, and the expression of the neural crest markers analysed. The injected side is indicated by an arrowhead. (A-D) XsnailZnFGR: dominant-negative of the Snail zinc fingers. Note that the dominant negative construct inhibited the expression of all the neural crest markers analysed. (E,F) Rescue of XsnailZnFGR by XsnailGR: both mRNAs were injected in equivalent amounts and analysed as previously described. Note the normal expression of the neural crest markers in the injected side. (G,H) SnailN-GR: dominant-negative using the Snail N-terminal domain. Note that the dominant-negative constructs inhibited the expression of all the neural crest markers analysed. (I,J) Rescue of XsnailNGR by XsnailGR: both mRNAs were injected in equivalent amounts and analysed as previously described. Note the normal expression of the neural crest markers in the injected side. Image published in: Aybar MJ et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai1 LOC108702592, sna, snail, snail1, xSna, Xsnail Xenopus Throughout unspecified stage neural crest embryo blastomere Image source: Published Larger Image Printer Friendly View

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