Xenbase Image ID: 2457

Fig. 3. Organiser gene expression in GR-Sia injected embryos induced at various stages. Embryos were injected ventrally with 50 pg/bl GR-Sia mRNA and 400 pg/bl LacZ RNA at the four-cell stage and DEX (10 μM) was either omitted (−DEX), or added 15 min before stage 9 (9−), or at stage 9.5. β-Galactosidase activity was revealed in red, and embryos were then processed for in situ hybridisation. For dkk1 and frzb, embryos were manually bisected prior to hybridisation. In all panels, the natural dorsal side is right. For otx2, the animal view (otx2/an) reveals the extent of the anterior neural plate. All dorsal genes examined, belonging to head and trunk classes, were robustly induced when DEX was added at stage 9−, while treatment at stage 9.5 provoked no or very weak induction. Image published in: Kodjabachian L and Lemaire P (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue frzb.S fiz, fre, frp-3, Frzb-1, frzb-pen, frzb1, fzrb, os1, sfrp3, srfp3, xfrzb X. laevis Throughout NF stage 11 endomesoderm dorsal marginal zone dorsal anterior involuted dorsal mesoderm involuted ventral mesoderm blastopore lip dkk1.S dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 11 endomesoderm involuted dorsal mesoderm dorsal marginal zone otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 11 dorsal dorsal marginal zone ectoderm upper blastopore lip animal cap gsc.S goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 dorsal marginal zone dorsal involuted dorsal mesoderm blastopore lip upper blastopore lip mesoderm chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 11 mesoderm dorsal marginal zone dorsal involuted dorsal mesoderm upper blastopore lip not.L flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Throughout NF stage 11 involuted dorsal mesoderm upper blastopore lip dorsal axial mesoderm dorsal marginal zone presumptive paraxial mesoderm Image source: Published Larger Image Printer Friendly View

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