Xenbase Image ID: 24576

Fig. 2. Expression pattern of Coco mRNA during Xenopus development. (A) Whole-mount in situ hybridisation of Coco, Bmp4 and VegT mRNA in the egg. Expression of Coco was also compared to Vg1 by RT-PCR. (B) Coco is expressed strongly in the animal pole at the 2-cell, 8-cell stages and at stage 8. (C) Coco is strongly expressed maternally and then is downregulated at the post-gastrula/pre-neurula stages. In contrast Cerberus is first expressed at stage 9 and then downregulated after the onset of neurulation. (D,E) At gastrula stage Coco is detected at high levels in the ectoderm and marginal zones by (D) whole-mount in situ hybridisation and (E) as seen by RT-PCR at much lower levels in the vegetal pole. ODC was used as a loading control for the RT-PCR. * indicates the organizer. Image published in: Bell E et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue dand5.S 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco X. laevis Sometime during NF stage 2 (2-cell) to NF stage 8 animal hemisphere vegt.L antipodean, brat, tVegT, vegt-a, vegt-b, xombi X. laevis Throughout unfertilized egg stage vegetal hemisphere dand5.S 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco X. laevis Throughout NF stage 10 to NF stage 12.5 animal cap dorsal marginal zone ventral marginal zone vegetal hemisphere bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout unfertilized egg stage animal hemisphere dand5.S 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco X. laevis Throughout unfertilized egg stage animal hemisphere Image source: Published Larger Image Printer Friendly View

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