XB-IMG-24686
Xenbase Image ID: 24686
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Fig. 3. In the retina, Xseb4R is expressed in retinoblasts and undifferentiated postmitotic neurons. (A-D) In situ hybridisation showing the spatio-temporal expression pattern of Xseb4R on retinal sections at various developmental stages. (A) At stage 28, Xseb4R is expressed in the whole presumptive neural retina that contains dividing retinoblasts (arrow). (B) At stage 32, Xseb4R is expressed in the whole neural retina containing mainly dividing precursor cells (arrow). (C) At stage 34, Xseb4R is most strongly expressed in the lens (arrowhead) and in the peripheral region of the neural retina containing dividing precursors (arrow). (D) At stage 37, Xseb4R expression is restricted to the CMZ (arrow) and the lens (arrowhead). (E-G) Staining for BrdU uptake (green; F) and Xseb4R expression (blue; E) at stage 39. Double staining (G) shows that BrdU-positive cells in the peripheral CMZ are Xseb4R negative (green arrowhead). In the central CMZ, BrdU-positive cells also express Xseb4R (black arrowhead). Some cells are BrdU negative and stained with Xseb4R (arrow). Scale bar in A: 50 μm. (H-J) Retinoblasts of stage 34 embryos were transfected at stage 18 with Xseb4R-Flag DNA. On sections, immunostaining was performed with an anti-FLAG antibody revealing the presence of XSEB4R-FLAG (red; H). (I) Higher magnification of this retinoblast. Hoescht staining was performed to visualise the nucleus. (J) Double staining showing that XSEB4R-FLAG is mainly cytoplasmic. The nucleus (arrow) and cytoplasm (arrowhead) are indicated. Scale bar in H: 30 μm. Image published in: Boy S et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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