XB-IMG-24689
Xenbase Image ID: 24689
|
Fig. 6. Xseb4R is involved in the regulation of primary neurogenesis in Xenopus embryos (A-C) Dorsal views of embryos injected with control lacZ mRNA (A), 200 pg of Xseb4R mRNA plus lacZ mRNA (B), or 50 pg of Xseb4R mRNA plus lacZ mRNA (C), and stained for N-tubulin expression (dark blue). β-galactosidase activity is shown in light blue. Whereas the high dose (200 pg) of Xseb4R represses N-tubulin expression on the injected side (arrow in B), the low dose (50 pg) leads to the expansion of the N-tubulin domain (arrow in C). (DG) Dorsal views of embryos after injection of the fusion construct Xseb4R-GR. Induction with dexamethasone was performed between stage 9.5 and 10 (D), or between stage 10.5 and 11 (E). F and G show non-induced control embryos. Whereas induction of Xseb4RGR between stage 9.5 and 10 represses N-tubulin expression in the injected side (arrow in D), induction of Xseb4R-GR between stage 10.5 and 11 leads to expansion of the N-tubulin domain (arrow in E). (H,I) Dorsal views of embryos after injection of control Mo (H) or Xseb4R Mo1 (I). Xseb4R Mo1 leads to a repression of N-tubulin expression in the injected side (arrowheads in I). (J) Effects of various doses of control Mo and Xseb4R Mo1 on N-tubulin expression. Injections of 10 or 20 ng of Xseb4R Mo1 lead to a decrease of N-tubulin expression compared with control embryos. Image published in: Boy S et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published
Larger Image Printer Friendly View |