Xenbase Image ID: 24699

Fig. 2. The effects of overexpression of CTGF resemble those caused by inhibition of WNT signalling. (A) Overexpression of CTGF (bottom embryo) causes a shortening of Xenopus embryos. Three independent experiments were performed with similar results each time. (B,C) Compared with controls (B), CTGF-injected embryos have an enlarged cement gland (C). (D-K) Unilateral overexpression of CTGF causes changes in gene expression that resemble those caused by inhibition of WNT signalling. (D) The expression domain of Otx2 is expanded. (E) CTGF causes a down-regulation of the future hindbrain domain of Pax6 and slightly expands the anterior domain. (F) CTGF causes down-regulation of N-tubulin in the neural plate. (G) The expression domain of Xsox3 in the neural plate is expanded. (H,I) Compared with the control side of the embryo (H), CTGF causes down-regulation of XSox3 in the dorsolateral placode (I). (J) CTGF causes down-regulation of Slug. (K) The muscle-specific gene myosin light chain 1 (MLC1) is down-regulated by Ctgf. I: injected; NI: Not injected. The injected sides of embryos are identified by pale blue lacZ staining. Image published in: Mercurio S et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 18 neuroectoderm anterior neural fold pre-chordal neural plate pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 13 to NF stage 21 neural tube sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 to NF stage 21 neural plate snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 13 to NF stage 21 neural crest mlc1.L LOC108711162, lvm, mlc, mlc1-3 X. laevis Throughout NF stage 13 to NF stage 21 somite tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Sometime during NF stage 18 to NF stage 19 neural plate Rohon-Beard neuron neuroectoderm Image source: Published Larger Image Printer Friendly View

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