Xenbase Image ID: 25040

Fig. 7. Neural-crest-specific expression of PTK7 and dsh constructs. Two-cell stage embryos were injected in one blastomere with plasmids containing a minimal slug promoter driving either the expression of GFP (A,B), wild-type PTK7 (C,D) or δkPTK7 (E,F). (A-F) Tadpole stage embryos analyzed by twist in situ hybridization. The injected side is presented in the right panel (B,D,F). (A,B) Embryo injected with 100 pg slug-GFP plasmid. (C,D) Embryo injected with 100 pg slug-PTK7 plasmid. (E,F) Embryo injected with 100 pg slug-δkPTK7. Arrowhead in F indicates the inhibition of neural crest migration. (G) Graph summarizing the percentage of migrating neural crest cells in five independent injection experiments. The number of injected embryos is indicated on each column. (H,I) Embryo injected with 50 pg slug-δDEP. (J-O) Embryo injected with 50 pg slug-PTK7 together with 50 pg slug-δDEP (J,K) or 50 pg slug-dsh (L,M) or 50 pg slug-δPDZ (N,O). (P) Graph summarizing three dsh and PTK7 co-injection experiments. The number of injected embryos is indicated on each column. Image published in: Shnitsar I and Borchers A (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist Xenopus Throughout NF stage 35 and 36 neural crest cranial neural crest trigeminal ganglion pharyngeal arch mandibular arch hyoid arch branchial arch mesenchyme neuroectoderm Image source: Published Larger Image Printer Friendly View

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