Xenbase Image ID: 25096

Figure 1. Expression of FRG1 transcript and protein during development. A: Analysis of Xenopus laevis frg1 transcript levels across developmental stages using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). B-G: In situ hybridization using the Xenopus laevis frg1 transcript at stage (St.) 26 (B), St. 32 (C), and St. 38 (E,F). Sense frg1 controls are shown for St. 32 (D) and St. 38 (G). Arrowheads point to migrating hypaxial muscle. H-J: FRG1 immunostaining on St. 18 (H), St. 24 (I), and St. 38 (J) embryos showing FRG1 antibody (upper) and secondary antibody alone control (lower). K,L: Stage 30 FRG1 immunostained embryos were sectioned sagitally (K) or transversely (L). Arrows point to aligned nuclei in the somite stained with FRG1 antibody. np, neural plate; ps, pronephric sinus; nt, neural tube; nc, notochord; is, intersomitic region. Image published in: Hanel ML et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue frg1.L X. laevis Throughout NF stage 26 eye head paraxial mesoderm frg1.L X. laevis Throughout NF stage 32 eye branchial arch paraxial mesoderm pharyngeal arch head region frg1.L X. laevis Throughout NF stage 37 and 38 mesoderm posterior cardinal vein hypaxial muscle pronephric sinus paraxial mesoderm head region frg1.L X. laevis Throughout NF stage 18 neural plate neuroectoderm frg1.L X. laevis Throughout NF stage 24 eye head somite neural tube frg1.L X. laevis Throughout NF stage 37 and 38 eye head somite frg1.L X. laevis Throughout NF stage 29 and 30 myotome neural tube notochord intersomitic region Image source: Published Larger Image Printer Friendly View

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