Xenbase Image ID: 2525

Fig. 5. Notch signaling is responsible for the initial dorsal restriction and mosaic expression of FoxI1e. (A) Maternal Xotch mRNA is depleted to 20–25% with 15 ng of thioate-modified DNA oligo. The level of Xotch remains low in the blastula and gastrula. (B) FoxI1e expression is 2- to 3-fold upregulated by depletion of Xotch. In situ hybridization shows an expansion of FoxI1e expression (C). (D) Injection of the constitutively active Notch Intracellular Domain (NICD) causes downregulation of FoxI1e relative to controls, and injection of the dominant negative construct Su(H)-DBM causes an upregulation of FoxI1e (E). (F) NICD upregulates the Notch target ESR-1, and Su(H)-DBM downregulates it. (G) In situ hybridization for FoxI1e comparing control and Su(H)-DBM injected embryos at stage 9.5 indicates that more, and in the most severe cases, all of the sensorial-layer animal cap cells express FoxI1e. Dorsal is to the right in all embryos shown. Scale bars represent 200 μm. Image published in: Mir A et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxl1 XFD-2 Xenopus Sometime during NF stage 9 to NF stage 10 ectoderm animal hemisphere dorsal Image source: Published Larger Image Printer Friendly View

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