XB-IMG-2544
Xenbase Image ID: 2544
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Fig. 6. RA signalling is required in pronephric precursors. A–C: XSMP-30 expression in embryos injected in the two dorsal blastomeres at the 4-cell stage with XCyp26 mRNA and β-galactosidase encoding mRNA as a lineage tracer. X-gal staining is revealed in blue and XSMP-30 expression in purple. When XCyp26 overexpression is restricted to the dorsal part of the embryos, XSMP-30 expression is not affected (B) whereas it is severely reduced when XCyp26 expression encompasses the pronephric primordium (arrowhead in panel C). An uninjected embryo is shown in panel A. D: Experimental design for the transplantation assay. E–G: Grafted embryo cultured to the tailbud stage 28. Lateral view of the anterior region showing localization of RLDx (red, F) and GFP/dnRAR (green, G) expressing cells. H–M: Grafted embryo cultured to the tadpole stage 38. Lateral view of the anterior region showing localization of RLDx (red, I) and GFP/dnRAR (green, J). Panels K, L and M present the same transverse histological section in the pronephric tubules area showing dapi staining (K), RLDx (L) and GFP (M) localization. Tubules contain RLDx but not GFP/dnRAR cells. In panels E–J, anterior is on the right and dorsal up. Image published in: Cartry J et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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