XB-IMG-2550
Xenbase Image ID: 2550
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Fig. 1. Dose-dependent effects of B56ε depletion on eye development. (A) Whole mount in situ hybridization showing the expression of rx (upper panels) and six3 (lower panels) in stage 20 control embryos, 2.5 ng εmor injected embryos, 5 ng εmor injected embryos, and 7.5 ng εmor injected embryos. εmor was injected into two dorsal animal blastomeres at the 8-cell stage. (B) Western blot showing the effects of εmor and 5mis on the translation of B56ε-FLAG. Myc-EGFP was used as a control for injection and loading. Morpholinos (5 ng) were injected into one of the dorsal animal blastomere at the 8-cell stage. Subsequently, a mixture of B56ε (200 pg) and Myc-EGFP (50 pg) was injected into the same blastomere. Injected embryos were harvested at the late gastrula stage. (C) Whole embryo phenotypes at the tadpole stage showing the effect of morpholinos injection on early embryonic development. Embryos were either uninjected (top), or bilaterally injected with εmor (5 ng, middle left; 7.5 ng, lower left), or injected with 5mis (5 ng, middle right; 7.5 ng, lower right) into both dorsal animal blastomeres at the 8-cell stage. (D) Whole mount in situ hybridization showing the expression of rx (upper panels) and six3 (lower panels) in stage 18 control embryos, 5 ng of 5mis injected embryos, and 7.5 ng of 5mis injected embryos. 5mis was injected into two dorsal animal blastomeres at the 8-cell stage. Note that less than 30% of embryos injected with 7.5 ng of 5mis exhibited fused rx or six3 expression at this stage. The rest of embryos were normal (not shown). Image published in: Rorick AM et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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