Xenbase Image ID: 2551

Fig. 2. B56ε plays a direct role in the regulation of rx expression. (A) RT-PCR showing that maternal depletion of B56ε (oocyte injection) reduced the expression of dorsal specific genes (dickkopf-1, goosecoid, cerberus, and Xenopus nodal related3 (xnr3)) and enhanced the expression of a ventral-specific gene (sizzled) at stage 11. Injection of 7.5 ng of εmor into two dorsal animal blastomeres at the 8-cell stage did not affect the expression of above genes. ODC was used as the loading control. (B) Whole mount in situ hybridization showing that the expression of chd in a control embryo and an embryo injected with 7.5 ng of εmor. (C) Whole mount in situ hybridization showing the expression of rx in a stage 18 uninjected embryo, an embryo injected with 0.5 ng of εmor, an embryo injected with 1 ng of εmor, and an embryo injected with 1.5 ng of εmor. εmor was injected into one of A1 blastomeres at the 32-cell stage. Lineage tracer, n-β-gal, was co-injected with εmor to indicate the side of injection. (D) Whole mount in situ hybridization showing the expression of rx (stage 12.5), sox3 (pan-neural, stage 12.5), otx2 (pan-forebrain, stage 12.5), and pax2 (midbrain, stage 14), arx (diencephalons, stage 14), and eomesodermin (telencephalon, stage 26/27) in control embryos (upper panels) and embryos injected with εmor (4.5 ng, lower panels). The right side was injected as indicated by the Red-gal staining. Embryos were injected unilaterally at the 8-cell stage. Image published in: Rorick AM et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue eomes eomesodermin, tbr2, xeomes Xenopus Sometime during NF stage 26 to NF stage 27 telencephalon forebrain neuroectoderm chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 18 dorsal marginal zone arx.S arx-a, arx-b, ct121, issx, mrx29, mrx32, mrx33, mrx36, mrx38, mrx43, mrx54, mrx76, mrx87, mrxs1, prt5, xARX X. laevis Throughout NF stage 14 diencephalon forebrain neuroectoderm sox3 Sry, Xlsox3, xSox3, Xtsox3 Xenopus Throughout NF stage 12.5 neuroectoderm otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 12.5 forebrain eye primordium anterior neural fold neuroectoderm pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 14 brain rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 12.5 eye primordium anterior neural fold rax rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 12.5 eye primordium Image source: Published Larger Image Printer Friendly View

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