Xenbase Image ID: 2554

Fig. 5. The PI3K/Akt pathway is required for IGF1-induced EFTFs. (A) Western blot showing that εmor injection blocked Akt phosphorylation induced by IGF1, but had no effect on Akt phosphorylation induced by P110. In addition, εmor had no effect on IGF1-induced ERK phosphorylation. εmor (10 ng) and RNA encoding IGF1 (2 ng) or P110 (1 ng) were injected sequentially, with εmor being injected at the 1-cell stage and RNAs being injected at the 4-cell stage. Animal caps were dissected at stage 8/9 and harvested at stage 13. (B) RT-PCR results showing LY294002, a PI3K inhibitor, blocked IGF1-induced expression of rx, lhx2, pax6, and six3, without affecting IGF1-induced otx2 and sox3 expressions in animal cap assay. IGF1 was injected as described above. Caps were dissected at late blastula stage and harvested at stage 13. Some caps were exposed to LY294002 from stage 9. (C) δp85 (2 ng) and dnAkt (2 ng) blocked IGF1 (2 ng) induced rx (upper panels), but not IGF1-induced otx2 (middle panels) and sox3 (lower panels) in whole embryos. One of the dorsal animal blastomeres was injected at the 8-cell stage. The right side was injected. Image published in: Rorick AM et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox3 Sry, Xlsox3, xSox3, Xtsox3 Xenopus Throughout NF stage 13 neuroectoderm otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 Xenopus Throughout NF stage 13 brain rax rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 13 eye primordium Image source: Published Larger Image Printer Friendly View

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