Xenbase Image ID: 2557

Fig. 8. B56ε is required for the expression of endogenous Hh target genes. (A) Whole mount in situ hybridization showing the expression of shh (upper panels) and ptc-1 (lower panels) was reduced by εmor (3.5 ng) injection at stage 14/15. The reduced shh and ptc-1 expression was partially rescued in embryos injected with εmor and ε-c (100 pg). (B) RT-PCR showing εmor (3.5 ng) injection reduced ptc-1, foxA2, and shh expression from late gastrula stage. ODC was the loading control. (C) The expression of ptc-1, ptc-2, foxA2, and shh in tadpole stage control embryos (left column) and embryos injected with 3.5 ng of εmor (right column). Embryos were bilaterally injected at the 8-cell stage. Image published in: Rorick AM et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Throughout NF stage 28 cloaca spinal cord floor plate visceral pouch foregut shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 14 to NF stage 15 dorsal neural plate pre-chordal neural plate notoplate ptch2.L patched 2, patched-2, ptc-2, ptc2, ptch2-a, ptch2-b, xptc 2, xptc-2 X. laevis Throughout NF stage 28 neural tube ptch1.S patched, patched1, Ptc-1, Ptc1, X-ptc1, XPtc1 X. laevis Sometime during NF stage 14 to NF stage 15 ectoderm neuroectoderm shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 28 notochord floor plate dorsal ptch1.S patched, patched1, Ptc-1, Ptc1, X-ptc1, XPtc1 X. laevis Throughout NF stage 28 somite dorsal tail region Image source: Published Larger Image Printer Friendly View

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