Xenbase Image ID: 26005

Fig. 2. ZFPIP is maternally and zygotically expressed during Xenopus laevis development. RT-PCR experiments using Xenopus laevis embryo RNA extracts show that ZFPIP mRNAs are present in stage 1 and then decrease until stage 12. Transcription of mRNAs is enhanced at stage 17 (A). The ZFPIP protein is maternally present in oocytes. The amount of protein is then diluted during the cleavage phase. The α-PCNA antibody is used as an internal quantitative control (B). Whole mount immunohistochemistry assays are performed on Xenopus laevis embryos using an α-ZFPIP or preimmune serum at stages 5, 8, 20 and 39. The pictures show that ZFPIP is mostly present in the animal pole of blastula and progressively localises in developing neural tissues (C). Western blots confirm that the protein is preferentially localised in the animal pole. AP, VP and E correspond respectively to animal pole, vegetal pole and embryo protein extracts (D). Nuclear localization of endogenous ZFPIP or hZFPIP-FLAG protein is observed in embryos or in hZFPIP mRNA injected embryos. The immunohistochemistry is performed with α-ZFPIP or α-FLAG antibodies on Xenopus laevis embryos explants (stage 20) (E). Image published in: Laurent A et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue znf462.S xZFPIP, Zfp462, ZFPIP, Zinc Finger Pbx1 Interacting Protein X. laevis Throughout NF stage 5 (16-cell) to NF stage 8 animal hemisphere znf462.S xZFPIP, Zfp462, ZFPIP, Zinc Finger Pbx1 Interacting Protein X. laevis Throughout NF stage 20 neural plate anterior neural fold neuroectoderm cranial neural crest neural crest eye primordium znf462.S xZFPIP, Zfp462, ZFPIP, Zinc Finger Pbx1 Interacting Protein X. laevis Throughout NF stage 39 head region otic vesicle eye cardiac mesoderm heart primordium spinal cord somite paraxial mesoderm nervous system tail region olfactory region Image source: Published Larger Image Printer Friendly View

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