Xenbase Image ID: 26052

Fig. 2. Expression of the 152 bp distal enhancer is premature compared to endogenous Xath5 expression and an additional 100 bp prevents premature transgene expression. (A–B) pG1 X5 3.3 kb and (C–D) X5 distal 1 kb embryos do not begin expressing GFP in the retina until after stage 22. (E–F) The X5 distal 152 bp mult transgenic embryos express GFP in the retina at both stage 22 and stage 32/33. Addition of 100 bp of sequence 5′ to the distal enhancer prevents premature expression (G–H). Each embryo was imaged at stage 22 and again at stage 32/33. Marks gut autofluorescence. (I) Deletion constructs were assayed for retinal GFP expression at stage 22 identifying a 100 bp region that inhibits precocious expression (marked by bracket). + strong expression, −/+ very weak expression, − no expression. Image published in: Willardsen MI et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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