Xenbase Image ID: 26067

Fig. 2. Sox3 activates geminin and sox2 expression in the absence of protein synthesis. (A) WISH of stage 12 embryos injected with sox3, sox3-VP16, or sox3-EnR mRNA (200–800 pg) at the 1-cell stage. Sox3 and Sox3-VP16 expand expression of sox2 and gem (arrows) and Sox3-EnR induces gem in stage 12 embryos. Images are ventral view with the anterior to the top. (B) High levels of Sox2 and Sox3 induce expression of gem in ectodermal explants. RT-PCR analysis of ectodermal explants for sox2, gem and ef1α expression from embryos injected with noggin (25 pg), sox2, sox3 (500 or 800 pg) and sox3-VP16 (200 pg). Sibling embryos were stage 12. (C) Sox3-GR induces expression of sox2 (Dex, n = 22/30; Dex + CHX, n = 12/20) and gem (Dex, n = 31/35; Dex + CHX, n = 46/54). (D) Sox2-GR induces gem in the absence of protein synthesis (Dex, n = 25/43; Dex + CHX, n = 35/47). WISH of embryos injected with either Sox2-GR or Sox3-GR (400 pg) and incubated without or with Dex (1 μM), CHX (10 μM), or Dex + CHX at either stage 9.5 or 11 and collected at stage 12. Hatched rectangle indicates region of flat mount high magnification shown below whole embryo images. Image published in: Rogers CD et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 Xenopus Throughout NF stage 12 dorsal neuroectoderm gmnn gem, geminin Xenopus Throughout NF stage 12 dorsal Image source: Published Larger Image Printer Friendly View

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