Xenbase Image ID: 26069

Fig. 4. Sox3, Sox2 and Sox3-VP16 inhibit Xvent2 expression and epidermal formation. (A) WISH for epi-k and Xp63 in embryos injected with 400–800 pg of sox3 or sox2 mRNA with lacZ (100 pg) mRNA into one of 2-cells. Uninjected (UI) side in top row, injected (I) side in bottom with arrow indicating injected area. Anterior is to the left and dorsal to the top. (B) Sox3, sox3-EnR, or sox3-VP16 mRNA (200–400 pg) was injected with lacZ mRNA into one of 2 blastomeres and gastrula or neurula embryos were assayed for bmp4, Xvent2 or epi-k expression by WISH. Bmp4 and Xvent2 (st. 12) embryos are animal pole view with the dorsal side to the top and epi-k embryos (st. 17) are a lateral view with anterior to the left. (C) WISH of stage 12 embryos injected with Sox3-GR into one blastomere of 2-cell embryos and untreated or treated with Dex at stage 9.5. Embryos are an animal pole views with dorsal to the top. (D) Diagram of BMP positive feedback loop, BRE-luciferase construct and relative luciferase activities in bar graph form. One-cell embryos were injected in the animal pole with vector or BRE-luciferase with sox3, sox2, sox11, sox3-EnR (400 pg), or sox3-VP16 mRNA (200 pg) and 50 pg CABR mRNA (top: lanes 3–9, and bottom: 2–5). The graph represents one experiment done in triplicate. Horizontal lines represent standard deviations. *p < 0.05 indicates values that differ significantly from lane 2 and **p < 0.01 indicates values that differ significantly from lane 6, Student’s t-test. Image published in: Rogers CD et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue bmp4.L bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 12 animal hemisphere ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 12 animal hemisphere tp63.L ket, np63, p63, tp73l, trp63, Xp63 X. laevis Throughout NF stage 17 cement gland primordium anterior neural fold epidermis ventral krt12.4.L ck81, cytok, cytokeratin, E-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, epi-keratin, epK, EpK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 17 epidermis ectoderm non-neural ectoderm Image source: Published Larger Image Printer Friendly View

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