XB-IMG-26093
Xenbase Image ID: 26093
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Fig. 3. Retinoic acid downregulates XRDH10 expression. (A-L) Whole-mount in situ hybridization analysis of XRDH10 transcription at neurula (A,B,E-L) and tailbud (C,D) stage. Embryos are shown in anterior (A,B,E,F,K,L), lateral (C,D, insets) and dorsal (G-L) views. (A-D) Embryos were treated from stage 11 (A,B) or stage 16 (C,D) onwards with 0.05% DMSO as a control or with 5 µM retinoic acid (RA). Note that RA induces a significant reduction in XRDH10 expression. (E,F) Embryos were microinjected into the animal pole at the four-cell stage with 2 ng XRALDH2 mRNA and treated from stage 11 onwards with 0.05% ethanol as a control (E) or 5 µM retinal (F). (G-J) Treatment from stage 11 onwards with the RA inhibitors disulfiram (10 µM) or citral (20 µM) causes an elevation of XRDH10 expression. (K,L) Embryos were animally injected into a single blastomere at the four-cell stage with 300 pg nlacZ mRNA as lineage tracer (red nuclei) alone (K) or together with 2 ng XCYP26A1 mRNA (L). Note that XCYP26A1 induces an upregulation of XRDH10 expression on the injected side (arrowhead). The indicated gene expression patterns were obtained in: A, 55/55; B, 22/31; C, 30/30; D, 37/37; E, 11/11; F, 16/17; G, 29/29; H, 49/49; I, 31/31; J, 54/57; K, 36/36; L, 48/53 embryos. (M) Negative-feedback regulation of RA biosynthesis. Image published in: Strate I et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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