Xenbase Image ID: 26096

Fig. 6. XRDH10 co-operates with retinol during head development. (A-D) Embryos were injected into the animal pole at the four-cell stage with the indicated mRNAs and treated with DMSO or retinol at stages 9-12. (A) DMSO-treated control embryo at tadpole stage. (B) Retinol (50 µM) induces microcephaly at the tadpole stage. (C) Injection of XRDH10 mRNA (1 ng into four blastomeres) and subsequent retinol treatment causes anencephaly. (D) XCYP26A1 mRNA (2.5 ng) partially restores eye and head structures in retinol and XRDH10-treated embryos. (E) Eye deficiencies induced by retinol and XRDH10, and dose-dependent rescue by XCYP26A1 mRNA in stage 40 embryos. (F) Control embryo at the tail bud stage after single injection of nlacZ mRNA. (G) Retinol (25 µM) leads to a slight reduction of the Rx2A-positive eye field (arrowheads). (H,I) In the retinol-treated embryos, XRDH10 mRNA (1 ng in one dorsal blastomere) causes a unilateral collapse of Rx2A expression (arrowhead in H), which is rescued by the co-injection of 2.5 ng XCYP26A1 mRNA (arrowhead in I). The indicated phenotypes were observed in: A, 24/24; B, 25/27; C, 28/45; D, 51/59; G, 13/15; H, 20/35; I, 10/13 embryos. Image published in: Strate I et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 23 eye egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 rhombomere R3 rhombomere R5 cranial neural crest Image source: Published Larger Image Printer Friendly View

Return to previous page