Xenbase Image ID: 26149

Fig. 4. Knockdown of Nemp1 by MOab disrupts eye development. (A) MOab inhibits splicing of nemp1 pre-mRNA. (Upper panel) Sequence alignment of exon 1–intron 1 splice junctions. Target sequences of MOa and MOb are underlined. (Lower panel) RT-PCR detection of unspliced transcripts in MOab (25 or 50 ng) or 5mmMO (50 ng)-injected embryos. RT−, RT-PCR without reverse transcriptase reaction. (B) Morphological appearances of injected embryos. MOab (Ba), 5mmMO (Bb), or MOab plus nemp1 mRNA (Bc) were injected together with nβ-gal mRNA as a tracer (red) into the animal pole region of both right blastomeres at the four cell stage. Injected doses (per embryo): MOab 20 ng; nemp1 mRNA, 200 pg. Upper panels, dorsal view (anterior is upwards); lower panels, right side view of the same embryo in the upper panels. (C) WISH analysis of 5mmMO or MOab-injected embryos at stages 13–14. Arrowhead, injected side. Image published in: Mamada H et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 13 to NF stage 14 neuroectoderm eye primordium tbx3.L tbx3-a, tbx3-b, Xltbx3, xtbx3 X. laevis Throughout NF stage 13 to NF stage 14 neuroectoderm eye primordium pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Sometime during NF stage 13 to NF stage 14 neuroectoderm eye primordium neural plate chordal neural plate six3.L hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 X. laevis Throughout NF stage 13 to NF stage 14 neuroectoderm eye primordium otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Sometime during NF stage 13 to NF stage 14 neuroectoderm eye primordium anterior neural fold sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 13 to NF stage 14 neuroectoderm neural plate pre-chordal neural plate chordal neural plate Image source: Published Larger Image Printer Friendly View

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