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Fig. 6. Confocal microscopic analysis of subcellular localization of Nemp1. Subcellular localization was analyzed by immunostaining as shown in Fig. 1D. C-terminally Myc-tagged deletion constructs of Nemp1 were analyzed by immunostaining (red). Nuclear DNA was stained with SytoxGreen (green). Eye-reducing activities and reduction of rax expression of the Nemp1 deletion constructs based on the results in Figs. 5B, C, respectively, were shown in the right of each panel; −, no activity; +, weak activity; ++, moderate activity; +++, strong activity. nd: not determined. Scale bars: 5 μm. Image published in: Mamada H et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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