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Fig. 1. Expression pattern of Xenopus laevis Irx genes. (A1–5) Vegetal views at early gastrula show that Irx1 (A1) and Irx3 (A3) are expressed in two dorso-lateral mesodermal bands (arrowhead). In addition, Irx1 and Irx2 are expressed in the presumptive neural ectoderm (A1 and A2, insets). (B1–5) Dorsal views of early neurula embryos illustrating the similar expression domains of all Irx genes (n, notochord). Arrowheads in (B1) and (B2) point at the placodal expression of Irx1 and Irx2. (C1) Early neurula embryo double-stained for Six3 (purple) and Irx1 (cyan) genes. The expression patterns of these genes are separated a few cell diameters (arrowheads). (C2–C5) Early neurula embryo double-stained for Irx genes (purple) and Fezf2 (cyan). Inset in (C2) show a similar embryo double-stained for Irx1 (purple) and Fezf2 (cyan). Insets in (C3–5) show double in situs developed in a single colour (purple) for Fezf2 and Irx3 (C3), Irx4 (C4) and Irx4 (C5). All Irx genes show similar anterior border abutting Fezf expression (arrowheads). (D1, D2) Double staining for Irx1 and Irx2 (D1) or Irx3 (D2) also show that these genes share their anterior expression limit (arrowheads). (D3, D4). This border is rostral to Pax2, as shown in double-stained embryos for this gene and Irx1 (D3) or Irx3 (D4). Inset in (D3) show a sagittal section of a double-labelled embryo for Pax2 (purple) and Irx1 (cyan). Note that Irx1 is anterior to that of Pax2. (D5) The Irx anterior limit overlaps with the posterior expression of Pax6 in the forebrain. (E1–G5) At tailbud stage, in lateral views (E1–5) or in dorsal views (F1–5, G1–5) all Irx genes show similar expression patterns in the brain although with different intensities in different regions. This is more clearly seen in double-stained embryos for Irx genes (purple) and Krox20 (cyan) (G1–G5). Red, black and blue arrowheads point at the M/H boundary, rhombomere 3 (r3) and rhombomere 5, respectively. Green arrowheads and red arrows point at the otic vesicle and pronephros, respectively. Stg, stage.
