Xenbase Image ID: 26317

Fig. 2. Irx MOs causes antero-posterior neural defects. Dorsal views of Xenopus tropicalis embryos at early neurula (stg 14–15; A–R) or tadpoles (stg 42; S–X) injected with 10 ng of MOIrx1 (A, G, M, S), MOIrx2 (B, H, N, T), MOIrx3 (C, I, O, U), MOIrx4 (D, J, P, V), MOIrx5 (E, K, Q, W) or a mix of 2 ng of each MO (F, L, R, X). The MOs were co-injected with LacZ mRNA as a tracer. In all embryos, red or black arrowheads point at the injected or control side, respectively. (A–F) In Irx morphant embryos, Gbx2 is reduced and shifted caudally. (G–L). Impairment of Irx genes also caused Otx2 posterior displacement and Krox20 downregulation but does not affect Cad3 expression. (M–R) In contrast, Wnt4 expression in the spinal cord, as well as in the midbrain, is reduced. A caudal shift of the midbrain is also observed in some cases. (S–X) Later, all injected embryos show brain malformations and some of them eye defects. Image published in: Rodríguez-Seguel E et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gbx2.1 XGBX-2 X. tropicalis Throughout NF stage 14 hindbrain posterior placodal area chordal neural plate egr2 EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. tropicalis Throughout NF stage 14 rhombomere R3 rhombomere R5 wnt4 wnt-4, Xwnt-4, Xwnt4 X. tropicalis Throughout NF stage 14 neural plate midbrain cdx4 cad3, MGC130769, MGC68909, Xcad-3, Xcad3, Xtcad3 X. tropicalis Throughout NF stage 14 neural plate posterior otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. tropicalis Throughout NF stage 14 forebrain Image source: Published Larger Image Printer Friendly View

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