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XB-IMG-2695

Xenbase Image ID: 2695


Figure 2. xWnt6 mRNA is expressed during Xenopus organogenesis. A: Quantitative reverse transcriptase-polymerase chain reaction analysis shows the relative expression levels of Wnt6 mRNA. Wnt6 expression is normalized to histone H4 levels. B-K: xWnt6 mRNA localization determined by whole-mount in situ hybridization using a digoxigenin-labeled xWnt6-specific LNA probe. L-P: Control embryos hybridized with a digoxigenin-labeled nonspecific LNA control probe. Numerical figures indicate the developmental stage. Note, xWnt6 mRNA initially localizes to animal pole and neural fold tissues, then more generally throughout the ectoderm and later in epithelial tissues and in the somites, the developing eye and pronephros. ba, branchial arches; c, cloaca; ea, eye anlage; g, gills; h, heart; hm, hypaxial muscles; nf, neural folds; pa, pronephric anlage; pt, pronephric tubules; s, somites.

Image published in: Lavery DL et al. (2008)

Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons.

GeneSynonymsSpeciesStage(s)Tissue
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 6 (32-cell)animal blastomere
ectoderm
animal hemisphere
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisSometime during NF stage 18 to NF stage 19ectoderm
anterior neural fold
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 23somite
eye
pronephric mesenchyme
epidermis
head
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 28ectoderm
branchial arch
fin
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 33 and 34 to NF stage 35 and 36ectoderm
branchial arch
fin
pronephric nephron
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisSometime during NF stage 40 to NF stage 42pronephric nephron
ectoderm
branchial arch
cloaca
fin
hypaxial muscle
external gill
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 10ectoderm
animal blastomere
dorsal
animal hemisphere
wnt6.Swnt-6, XWnt-6, xWnt6X. laevisThroughout NF stage 13ectoderm
animal blastomere
animal hemisphere

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