XB-IMG-2697
Xenbase Image ID: 2697
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Figure 4. Wnt6 protein is expressed at different stages of development and is localized to the epidermis overlaying developing organs and at later stages to the pronephric tubules. A,B: Western blots showing both endogenous xWnt6 expression as well as overexpression of xWnt6 and mWnt6 in lanes two and three at 3 hr after injection (A) and 5 hr after injection (B). A,B middle panels: Immunodetection showing expression of myc-tagged proteins xWnt4 and xWnt5A at 2 hr and 5 hr after injection, respectively. A,B bottom panels: Immunodetection of ERK2, demonstrating relatively equal protein loading in each lane. C: Western blot showing xWnt6 protein expression at different stages of development. C bottom panel: Immunodetection of ERK2, demonstrating equal protein loading in each lane. D-F,H,I: Whole-mount immunocytochemistry staining using an anti-Wnt6 antibody. G: Control embryo stained without primary antibody and with secondary antibody alone. I: Insert showing close-up of xWnt6 protein expression in the pronephric tubules at stage 40. Numerical figures indicate developmental stage. e, eye; h, heart, p, pronephros; pt, pronephric tubules. Image published in: Lavery DL et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons.
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