Xenbase Image ID: 2739

Fig. 3. Siamois and Twin are required for head formation. (A) Twn-MO injected embryo. Small eyes were detected in 10.3% of injected embryos (3/29). (B) Sia-MO injected embryo. Small eyes were detected in 87.5% of embryos (42/48). (C) Twn-MO and Sia-MO co-injected embryos. 80% of embryos (28/35) lost head structures. (D) Co-injection of Twn-MO and Siamois mRNA rescued the Twn-MO phenotype to normal or mild dorsalization (big eyes in 42.9% (15/35) of the embryos). (E) Sia-MO and Twin mRNA coinjected embryo. Small eyes were detected in 14.8% of embryos (4/27). (F) Control uninjected sibling embryo for (A–E). (G) Sagittal histological section of the embryo shown in C. (H) Sagittal histological section of embryo shown in F. (I) Whole-mount in situ hybridization analysis of the anterior neural marker Otx2 in stage 26 embryo co-injected with Twin-MO and Siamois-MO. (J) Otx2 expression in control embryo at stage 26. fb, forebrain; mb, midbrain; hb, hindbrain; nt, neural tube; no, notochord; he, heart. Image published in: Ishibashi H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sia1.L sia, Siamois, Xsia X. laevis Throughout NF stage 26 brain heart forebrain midbrain hindbrain neural tube notochord otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 26 midbrain brain hindbrain Image source: Published Larger Image Printer Friendly View

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