XB-IMG-3101
Xenbase Image ID: 3101
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FIG. 1. Temporal expression of Notch-1, Delta-1, and Serrate-1 during pronephric development. (A) Schematic of a nonintegrated nephron.
Wastes are filtered from the glomus into the coelom where fluids are swept into the kidney tubules by the thin ciliated funnels
(nephrostomes). Any molecules not resorbed in the pronephric tubules are disposed of via the pronephric duct. (B) Expression patterns of
Delta-1, Serrate-1, and Notch-1 via whole-mount in situ hybridization between stages 21 and 34. Embryos are shown laterally with anterior
to the right and dorsal to the top. The pronephric anlage is located just posterior to the head and ventral to the anterior somites. Note the
dynamic expression patterns of both ligands within the pronephric anlage, as the expression pattern of Notch pathway components becomes refined during kidney morphogenesis. Red arrows indicate the region in which the pronephros is located, but no Delta-1 (d) or
Serrate-1 (e) expression can be detected at these stages. Black arrows mark the position of Delta-1, Serrate-1, or Notch-1 expression in the
pronephros. (C) Time-line summary of Delta-1, Serrate-1, and Notch-1 expression patterns determined by whole-mount in situ
hybridization. Note that Delta-1 expression is observed in the pronephric anlage (stage 19) prior to detection of Serrate-1 expression (stages
22 23). Unlike Serrate-1 expression, which continues to be expressed until tadpole stages, Delta-1 expression decreases and is no longer
detectable by stages 33 34. The expression of the receptor, Notch-1, is detected from the onset of kidney organogenesis (early tail bud) and
continues to be expressed until the pronephric kidneys are fully functional (stages 38 39). Image published in: McLaughlin KA et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V.
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