Xenbase Image ID: 3107

Figure 4. The Phenotype of the FCN Triple Morphant Is Specific to a Loss in BMP AntagonismAll panels are dorsal views of neurula embryos (st 14) with anterior to the top. Triple morphants were subsequently injected with pufferfish noggin mRNA (C, F, and I) and show substantial rescue of dorsal structures (sox2 [A–C], myoD [D–F], and shh [G–I]) when compared to triple morphants (B, E, and H) and uninjected sibling embryos (A, D, and G). The distribution of phenotypes seen in these rescue experiments is depicted in a bar graph (J). The vertical axis is the percent of embryos that show a particular phenotype. The horizontal axis shows the experimental groups and the phenotypes seen. The black bar depicts the percentage of embryos with a substantial neural plate, the dark gray bar depicts the percentage of embryos with a minor neural plate, and the light gray bar depicts the percentage of embryos with no neural plate that show only a ring of sox2 expression. We also tested the phenotype of the triple morphant for specificity by subsequently injecting BMP4,7 MOs (BMP MO), which should reduce BMP signaling. A partial rescue of the neural plate is seen (M) compared to triple morphants (L) and uninjected sibling control embryos (K). Image published in: Khokha MK et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. tropicalis Throughout NF stage 14 neural plate myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. tropicalis Throughout NF stage 14 presomitic mesoderm shh hedgehog, vhh-1, X-SHH, Xhh, xshh X. tropicalis Throughout NF stage 14 floor plate notochord Image source: Published Larger Image Printer Friendly View

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