XB-IMG-3269
Xenbase Image ID: 3269
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Fig. 5. Overexpression of Evi1 down-regulates Xbra and chordin expression but not other genes in the early Xenopus embryo. (A) Embryos injected with Evi1 mRNA failed to complete gastrulation. (B–F) Whole-mount in situ analysis of the indicated markers in embryos coinjected with Evi1 and β-galactosidase mRNA. In all cases, β-galactosidase activity is revealed with X-gal. (B, C) Vegetal views of injected embryos at early gastrula stage. A reduction of the expression of the mesodermal markers Xbra and chordin is seen in the injected area. (D–F) Anterior view (D) and dorsal views (E, F) of neurula stage injected embryos analyzed for the expression of the ectodermal EpK, the neural crest Slug and the neural Sox2 marker. Note that Evi1 mRNA injection has no effect on the expression of these markers. Image published in: Van Campenhout C et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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