Xenbase Image ID: 3308

Fig. 5. Notch signaling regulates the expression of Oct-1. A–C: the expression profile of Oct-1 in Xenopus embryo. All panels are dorsal views, anterior toward left. A: stage 17, late neurula. B: stage 25, early tailbud. C: stage 30, tailbud. A′–C′: transversal sections cut at the level of prospective hindbrain or hindbrain in panels A–C, respectively. a: anterior, p: posterior, e: eye, hb: hindbrain. D–M: whole-mount in situ hybridization analysis at stage 25. Oct-1 expression is expanded in the GR-NICD-injected (E: n = 33/45) and at-GRSu(H)-injected (L: n = 26/26) side of the brain indicated by a brace and reduced in the dn-Notch-injected (J: n = 17/30) but not dn-GRSu(H)-injected (M: n = 32/32) side of the brain. The activation of Notch at stage 20 did not expand the Oct-1 expression (F: n = 30/30). On the hand, the XHey-1 expression was increased or reduced by GR-NICD activated at stage 18 (G: n = 36/40) or dn-Notch (K: n = 30/42), respectively. The expression of vimentin was increased by GR-NICD (H: n = 32/39) and Oct1-GR (I: n = 34/40) activated at stage 18. The injected side in each embryo is indicated by nucβ-Gal staining (red color). The Oct-1 expression is not changed by injection of nucβ-Gal (D: n = 47/47). Image published in: Kiyota T et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pou2f1.S NF-A1, oct-1, oct1, OTF-1, pou2f1r, XOct1 X. laevis Throughout NF stage 25 hindbrain central nervous system eye primordium neural tube anterior neural tube neuroectoderm pou2f1.S NF-A1, oct-1, oct1, OTF-1, pou2f1r, XOct1 X. laevis Throughout NF stage 17 neural plate neuroectoderm anterior posterior pou2f1.S NF-A1, oct-1, oct1, OTF-1, pou2f1r, XOct1 X. laevis Throughout NF stage 29 and 30 brain hindbrain central nervous system neural tube eye primordium Image source: Published Larger Image Printer Friendly View

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