XB-IMG-3320
Xenbase Image ID: 3320
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Fig. 3. Depletion of dystroglycan leads to a drastic diminution of circonvoluted tubules. (A–D) Microinjections were performed unilaterally at the 4-cell stage with Dg-Mo1 + 2 (16 ng) in both left blastomeres. Embryos were fixed at stage 40 and then development of pronephros was analysed by immunochemistry staining with monoclonal antibodies 3G8 for tubules and 4A6 for ducts. (A, B) Whole-mount immunochemistry. The pronephric tubules (red arrowhead) and the anterior duct segment (blue arrowhead) in panel B show severe developmental defects in the injected side compared to control side in panel A. (C, D) Cryostat sections of these embryos at level of the pronephros area. A single and large 3G8-positive tubule is present in the injected side (D) whereas in the control several tubules are detected (C). (E–J) Expression of pronephros marker genes in Dg-Mo injected embryos. Embryonic stage is indicated in the top right of each panel. The expressions of the Pax-8 (E–H) and Pax-2 (I, J) genes were detected by whole-mount in situ hybridization on embryos injected unilaterally with Dg-Mo1 + 2 (16 ng). The expression of the Pax-8 gene is similar in the control (right side of the embryo in panels E–G) and injected sides at all developmental stages. In spite of unchanged Pax-8 expression, some defects in the pronephros area are observed in the injected side: the duct is shorter and enlarged in stage 24 embryo (panel F, green arrowhead). In the control side of stage 35 embryo three nephrostomes (panel G, red arrowheads) are detected, in contrast in the injected side only a single structure is observed (H). In stage 35 embryo, the expression of the Pax-2 gene is not modified in the injected side (J) compared to the control (I). Here again a single structure is observed in the injected side (J) compared to the three nephrostomes observed in the control side (I). Image published in: Bello V et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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