Xenbase Image ID: 33843

Figure 4. miR-427 Loss of Function Affects Mesodermal Patterning and Organizer Induction in Vivo(A) Ventralization of mesodermal explants by miR-427 depletion. Xenopus eight-cell-stage embryos were injected into both dorsal (DMZ) or ventral (VMZ) marginal blastomeres with 10 ng α-miR-427-Mo. Uninjected and Mo-injected DMZ and VMZ explants were dissected at stage 10 and assayed for expression of the indicated markers by RT-PCR analysis.(B) Whole-mount in situ hybridization for the organizer markers, chordin and goosecoid (purple staining). Embryos injected dorsally with LacZ RNA together with either α-miR-427-Mo or lefty mRNA or left untreated were stained at stage 10. Light-blue staining represents expression of the lineage tracer LacZ in the injected side of the embryo.(C) Decreased Nodal activity in miR-427-depleted embryos. Different doses of Xnr1 RNA, as indicated, were injected alone or together with α-miR-427-Mo (10 ng) into the animal pole of two-cell-stage embryos. Animal caps were examined at the gastrula stage (stage 11) for the expression of Xnr target genes by RT-PCR. Chd, chordin; gsc, goosecoid; ODC, ornithine decarboxylase; xbra, brachyury. Image published in: Rosa A et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 11 dorsal marginal zone mesoderm gsc goosecoid, gsc-a, gsc-b, Xgsc Xenopus Throughout NF stage 11 dorsal marginal zone mesoderm Image source: Published Larger Image Printer Friendly View

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