Xenbase Image ID: 37772

Fig. 2. Comparison of expression patterns of dnah5, dnah9 and dnah11 in ciliated embryonic tissues. (A–C) Immunohistochemistry using an antibody against acetylated alpha tubulin to highlight cilia. (A) Ciliated cells at the GRP, nephrostomes (B’), epidermis (B”) and tailbud (B’”) of the 2-day tadpoles, and (C) in stomach and small intestine. (D–L) Whole-mount in situ hybridization of staged embryos with dnah5 (D–F), dnah9 (G–I) and dnah11 (J–L) specific antisense probes. GRPs (A, D, G, J), outlined in dorsal explants by dotted lines, are shown in ventral view, anterior to the top. (B, E, H, K) 2-day tadpoles are oriented anterior to the left and dorsal up; blow-ups are indicated by dashed lines. Insets show frontal view of proctodeum (arrowhead). (C, F, I, L) Ventral views of stage 43/44 tadpoles. Proctodeum marked by arrowheads. Note that dnah5 and dnah9 are co-expressed in ciliated cells, with dnah9 showing markedly stronger signals, whereas dnah11 is only expressed in the proctodeum, tailbud and gastro-intestinal tract. Please note also, that dnah5 and dnah9 are restricted to the neural tube of the tailbud, whereas dnah11 is additionally expressed in the posterior wall. cnh, chordoneural hinge; fp, floor plate; nc, neuroenteric canal; no, notochord; s, stomach; si, small intestine. Image published in: Vick P et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue dnah5.L cild3, dnahc5, hl1, ktgnr, pcd X. laevis Throughout NF stage 18 ciliated epidermal cell gastrocoel roof plate roof plate ectoderm ectodermal cell otic region dnah9.S dnah9-A, L-R Dynein, Left-Right Dynein, LRD X. laevis Throughout NF stage 18 ciliated epidermal cell gastrocoel roof plate dnah5.L cild3, dnahc5, hl1, ktgnr, pcd X. laevis Throughout NF stage 33 and 34 ciliated epidermal cell pronephric nephrostome otic vesicle posterior neural tube floor plate chordoneural hinge neural tube dnah9.S dnah9-A, L-R Dynein, Left-Right Dynein, LRD X. laevis Throughout NF stage 33 and 34 ciliated epidermal cell pronephric nephrostome otic vesicle posterior neural tube floor plate epidermis proctodeum lateral plate mesoderm neural tube ectoderm neuroectoderm chordoneural hinge eye fin dorsal tail fin neural crest dnah11.L cild7, dnahbl, dnahc11, dnhbl, dpl11 X. laevis Throughout NF stage 33 and 34 posterior neural tube posterior wall of neurenteric canal chordoneural hinge ectoderm proctodeum neuroectoderm dnah5.L cild3, dnahc5, hl1, ktgnr, pcd X. laevis Throughout NF stage 43 esophagus small intestine stomach proctodeum ciliated epidermal cell alimentary system ectoderm pharyngeal arch mandibular arch hyoid arch branchial arch dnah9.S dnah9-A, L-R Dynein, Left-Right Dynein, LRD X. laevis Throughout NF stage 43 esophagus small intestine stomach proctodeum dnah11.L cild7, dnahbl, dnahc11, dnhbl, dpl11 X. laevis Throughout NF stage 43 esophagus small intestine stomach proctodeum pharyngeal arch alimentary system Image source: Published Larger Image Printer Friendly View

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