XB-IMG-37773
Xenbase Image ID: 37773
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Fig. 3. Knockdown of dnah9 in the epidermis inhibits ciliary motility. (A) Ventral injection of lineage tracer mRFP into the marginal regions of vegetal and animal right blastomeres resulted in specific labeling of right but not left epidermis at stage 32. (B) Tadpole motion assay. Stage 32 tadpoles were placed individually into six Petri dishes and monitored by standard (CCD) digital cameras at 25 fps. Movements were recorded via connected computers (PC) and calculated using a custom-made software. (C) Unilateral dnah9-MO-injection resulted in a very highly significant decrease of tadpole velocity on the injected side. (D–G) Ciliation of epidermal cells was unaffected in dnah9-MO-injected tadpoles. (D, E) Immunohistochemistry of stage 32 tadpoles using an antibody against acetylated tubulin (green) and red fluorescence from co-injected lineage tracer mRFP (red). Overlays reveal equal ciliation in specimens injected with control MO (D) and in dnah9 morphants (E). (F, G) Scanning electron micrographs of control MO (F) and dnah9-SB-MO-injected tadpoles. Targeted regions of skin are highlighted in (F) and (G) by overlay with injected lineage tracer (GFP). Higher power magnifications of targeted regions in (F’, F”, G’, G”) demonstrate equal ciliation in control-injected and morphant embryos. an, animal; d, dorsal; l, left; r, right; v, ventral; veg, vegetal. Scale bars represent 10 μm. Image published in: Vick P et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.
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