Xenbase Image ID: 40211

Fig. 4. Altering endogenous RA levels altered the expression of Hand1 and FoxF1 but had little effect on the expression of Nkx-2.5 or Xsal-1. Embryos were treated with ketoconazole (left column) to increase endogenous RA levels by inhibiting Cyp26 and DEAB or citral was used to lower endogenous RA levels by inhibiting RALDH2. The expression domain of Nkx-2.5 (A–D) was not noticeably altered by any of the treatments when compared to DMSO controls (B). In contrast, the expression of FoxF1 (E–H) was not obviously altered by treatment with ketoconazole (E) but both DEAB and citral (G and H) treatments reduced the expression domain of FoxF1 as compared to the DMSO control embryos (F). Similarly, the size of the Hand1 (I–L) domain was not clearly altered by ketoconazole (I) when compared to controls (J), but both DEAB and citral (K and L) diminished the expression domain. The expression domain of Xsal-1 was not altered by any of the treatments (M–P). Panels A–D show the anterior pole of the embryo, with dorsal toward the top; panels E–P show the left lateral side of the embryo, with dorsal toward the top, anterior at left. Image published in: Deimling SJ and Drysdale TA (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 20 lateral plate mesoderm hand1.L eHand, hand-1 X. laevis Throughout NF stage 20 mesoderm lateral plate mesoderm ventral nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 20 mesoderm cardiac mesoderm anterior sall3.L X-sal, Xsal-1, Xsal-3 X. laevis Throughout NF stage 20 lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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