Xenbase Image ID: 40626

Fig. 5. miR-124a overexpression inhibits cell proliferation during retinogenesis. miR-124a or control miRNA precursor (0.025 pmol) was co-injected with GFP mRNA into one dorsal-animal blastomere of a Xenopus embryo at the 8-cell stage, and cell proliferation was detected by BrdU incorporation. (A and B) Transversal section of a stage 33 embryo at the level of the eye showing that the number of proliferating cells (BrdU-positive, red) on the miR-124a overexpressed side (miR-124a) was much lower than that on the control side (C). All cell nuclei were counterstained with Hoechst 33258 (blue). Scale bar: 100 μm. (C) The ratio of proliferating retinal cells (BrdU-positive/Hoechst-labeled cells) in either the injected side (inj) or the control side (C) of embryos injected with miR-124a (pre-miR-124a) or control (pre-control) precursors. Data are expressed as means ± SE from twelve sections of four embryos. A significant difference (**P < 0.01 by one-way ANOVA followed by Duncan’s test) between the mean ratios between the injected and control sides was only detected for pre-miR-124a injected embryos. Image published in: Qiu R et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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