Xenbase Image ID: 40658

Fig. 6. Wnt/β-catenin activation, but not Sox2, promotes cell proliferation. (A-D) Electroporation with TCF3-VP16 (n=5, 493 cells), but not Sox2 (n=3, 1719 cells), significantly increases the proportion of BrdU+ cells (arrowheads) at stage 37, as compared with control GFP (n=4, 653 cells). (E) GFP, Sox2 and TCF3-VP16 were electroporated and proliferation assessed by flow cytometry at stage 37. Transfected cells display a GFP intensity above background, whereas non-electroporated retinas do not have any GFP+ cells (inset). (F) TCF3-VP16 transfection causes a 2-fold increase in the proportion of cells in the S/G2/M phases, as compared with Sox2 or GFP transfection, indicating a larger proliferating cell fraction (see inset). (G) Co-lipofection of the dominant-negative Sox2BD(-) does not inhibit BrdU incorporation caused by TCF3-VP16 at stage 41 (GFP, n=12, 2615 cells; Sox2BD(-), n=9, 670 cells; TCF3-VP16, n=11, 1539 cells; TCF3-VP16+Sox2BD(-), n=10, 1242 cells. Error bars indicate 95% C.I. *P<0.001 compared with GFP control. Image published in: Agathocleous M et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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