XB-IMG-40671
Xenbase Image ID: 40671
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Fig. 3. Results of representative loss and gain of XDppa2/4 function experiments. (A) Depletion of XDppa2/4 leads to defects during gastrulation and lack of neurulation. Injection of XDppaMO gives rise to a wide-open blastopore at stage 11.5. The blastopore remains open until stage 18 and neural fold formation is missing. Most of the embryos die before the end of neurulation and disaggregate. Co-injection of 50 ng XDppaMO and 600 pg mut-XDppa2/4 RNA rescues development and restores the wild type. As controls, unmanipulated embryos and embryos injected with control MO (ctrlMO) are shown. (B) Gain of function studies with XDppa2/4, mDppa2 or mDppa4 RNA result in comparable phenotypes. Overexpression of 400 pg XDppa2/4 reveals defects in blastopore closure. At stage 17, the embryos form a neural fold but still show an open blastopore. At tailbud stage, XDppa2/4 gain of function results in reduction of body axis, tail structures and open neural folds. Overexpressions of mouse mDppa2 (800 pg) and mDppa4 (100 pg) lead to similar phenotypes including failure of blastopore closure, open neural fold and axis reduction. Uninjected embryos are shown as controls. Image published in: Siegel D et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |