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Fig. 4. Whole mount in situ hybridization of gain and loss of function embryos. Embryos were injected with XDppa2/4, mDppa2, mDppa4 RNA or XDppaMO into both blastomeres at 2-cell stage. Whole mount in situ hybridization was performed at stage 11 for Xbra, BMP4, MyoD, Gsc, XSox2, Xema and XSox17a. Uninjected embryos or embryos injected with ctrlMO show the expected wild type expression pattern of marker genes at stage 11. Orientation of embryos is given. For the vegetal view the dorsal side is always located at top and for the lateral view the dorsal side is located at the left side. Image published in: Siegel D et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 marginal zone blastopore mesoderm bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 11 ventro-lateral marginal zone sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 11 marginal zone blastopore endoderm sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 11 ectoderm upper blastopore lip neuroectoderm gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 dorsal dorsal marginal zone foxi1.L ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 X. laevis Throughout NF stage 11 ectoderm animal cap myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 11 ventro-lateral marginal zone mesoderm Image source: Published Larger Image Printer Friendly View

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