Xenbase Image ID: 40674

Fig. 6. The SAP domain is dispensable for the gain as well as for the loss of function phenotypes. (A) Schematic representation of XDppa2/4 deletion constructs used in overexpression studies. Numbers represent positions of amino acids. The SAP domain is shown in yellow and the DCR domain in turquoise. L represents the linker region. (B) Results of overexpression of mutant transcripts. Vegetal view on uninjected control embryo at stage 11.5 and embryos injected with a total of 800 pg XDppa2/4, δSAP, δDCR, δSAPδDCR, DCR, SAP + L + DCR, aa1–65, aa66–240 and δN-term RNA into two blastomeres at the 2-cell stage. (C) Rescue of the loss of function (MO) phenotype by co-injection of mut-δSAP RNA. Dorsal view of embryos at stage 18. Embryos injected with 50 ng MO or with 650 pg mut-δSAP suffer from gastrulation defects and lack of neurulation. Neural fold formation is rescued by co-injection of 550 pg mut-XDppa2/4 or 600 pg mut-δSAP RNA. Image published in: Siegel D et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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