Xenbase Image ID: 40675

Fig. 7. Subcellular (A) and subnuclear (B) localization of XDppa2/4 deletion mutants. (A) Transfections of HeLa cell lines with indicated EGFP-fused XDppa2/4 deletion constructs show differences in subcellular localization. Constructs encoding EGFP alone or wild type XDppa2/4 were transfected as controls. (B) Only XDppa2/4 proteins with an intact SAP domain reveal chromatin association as shown by co-staining with histone H2B-mRuby (red) and DAPI (blue). (C) Results of subcellular localization experiments in comparison with phenotypes obtained after overexpression of the respective deletion constructs. A diffuse localization within the nucleus was interpreted as indication that the respective mutant is not associated with chromatin. Image published in: Siegel D et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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